Journal: bioRxiv

Article Title: Development of high-affinity, single-domain protein binders for neutralizing household allergens

doi: 10.1101/2025.08.03.668213

Figure Lengend Snippet: (a) Thermostability profile of AVA-1-1-C3 as a representative protein, as determined by differential scanning fluorimetry. (b-g) Cytotoxicity of primary human cells following 48-hour exposure to AVA-1-1-C3 or an equivalent concentration of bovine serum albumin (BSA), evaluated using the CellTiter-Glo® (CTG) luminescent cell viability assay. Cell types included human epithelial keratinocytes (HEK, b), human dermal fibroblasts (HDF, c), human follicle dermal papilla cells (HFDPC, d), human renal cortical epithelial cells (HRCEPC, e), human skeletal muscle cells (HSKMC, f), and human dermal microvascular endothelial cells (HDMEC, g). No significant differences in cell viability were observed. Data in (b-g) represents mean ± s.d. and are representative of two experimental replicates.

Article Snippet: For in vitro toxicity studies, primary cells including human dermal fibroblasts (HDF, Cell Applications, Cat# 106K-05a, lot 1632), human epidermal keratinocytes (HEK, Cell Applications, Cat# 102-05a, lot 2146), human dermal microvascular endothelial cells (HDMEC, PromoCell, Cat# C-12210, lot 483Z001.3), human skeletal muscle cells (HSKMC, Cell Applications, Cat# 150K-05a, lot 3507), and human renal cortical epithelial cells (HRCEpC, Promocell, Cat# C-12660, lot 501Z019.23).

Techniques: Concentration Assay, Cell Viability Assay

Journal: Current protocols in stem cell biology

Article Title: Derivation of telencephalic oligodendrocyte progenitors from human pluripotent stem cells

doi: 10.1002/cpsc.17

Figure Lengend Snippet: (A) Protocol scheme for differentiation of hESCs into telencephalic oligodendrocytes. (B) Immunophenotyping of hESC progeny during differentiation into oligodendrocytes. Undifferentiated hESC colony expressing Tra1-60, a human pluripotent stem cell marker. (C) Around day 20 of differentiation, neural precursor cells form typical rosette-like structures with expression of neural stem cell progenitor marker Nestin and rosette-associated marker ZO1 in the center of the rosettes. (D) In addition to positive staining for another rosette marker LIN28, homogenous expression of the forebrain-associated marker BF1 confirms the anterior identity of the NPCs (E) Patterned NPCs express early neuroectodermal markers such as Pax6 (F) On day 50 early OPCs appear in the culture and co-expressi Olig2 and Nkx2.2. (G) Late OPCs present on day 70 express O4 together with Olig2. (H) Later OPCs acquire the expression of the more mature O1 oligodendrocyte marker. (I) Using flow–cytometry, O4 expressing late OPCs can be purified from the culture on day 70, then exposed to terminal differentiation condition for two weeks. By day 85, cells will fully mature and express myelin basic protein (MBP). Scale bars represent 100μm.

Article Snippet: D1306) Primary and Secondary antibodies Anti-Olig-2 Antibody (1:500, EMD Milipore, cat. no. AB9610) Nkx2.2 antibody (1:100, DSHB, cat. no. 74.5A5) Nestin Mouse Antibody (1:500, Neuromics, cat. no. MO15012) Anti-TRA-1-60 Antibody (1:100, EMD Milipore cat. no. MAB4360) Anti-Pax-6 Antibody (1:500, BioLegend, cat. no. 901301) Anti-O4 Antibody (1:200, EMD Millipore, cat. no. clone 81 MAB345) Anti-O1 Antibody (1:200, EMD Millipore, cat.no. clone 59 MAB344) Anti-Myelin Basic Protein Antibody (MBP; 1:200, EMD Millipore, cat.no.

Techniques: Expressing, Marker, Staining, Flow Cytometry, Purification